@article {209,
	title = {FTIR characterisation and in vitroactivities of Bombacopsis buonopozense and Nauclea latifolia leaf extracts against bacteria isolated from cattle rumen waste and vegetable farm irrigation water},
	journal = {. UNIOSUN J. Sc.},
	volume = {1},
	year = {2016},
	pages = {38-46},
	abstract = {

Introduction: Symbiotic relationship between gut bacteria, antibacterial activity microbiota and animal models, as well as, between plantparasitic microbes and economic vegetable crops act as a veritable tool to test and provide information on the characteristic changes in health and disease. It provides a framework for control of pathogenic microbes. In this work, therefore, we sought to study the susceptibility of cattle rumen waste and vegetable farm irrigation water bacterial flora to aqueous and ethanolic leaf extracts of\&nbsp;Bombacopsis buonopozense\&nbsp;and\&nbsp;Nauclea latifolia\&nbsp;Sm.
Methods: Following cold extraction, phytochemical screening of the leaf extracts was done. Paper disc diffusion method was employed to assay for antibacterial activities of the leaf extracts, while commercial Ofloxacin (5μg), Ci p r o f l o x a c i n ( 5 μ g ) , Ge n t ami c i n ( 1 0 μ g ) , Chloramphenicol (10μg) and Cotrimoxazole (25μg) served as standard antibiotics. Predictions of the functional groups of bioactive compounds in the different fractions of the leaf extracts were made using FTIR spectroscopy.
Results: Approximately 94.87\% of the bacteria were sensitive (17-38 mm Inhibition Zone Diameter [IZD]) to the standard commercial antibiotics; while they were not sensitive to the test concentrations of both the aqueous and ethanol leaf extracts of B. buonopozense (0-13.5 mm IZD) and N. latifolia (0-11.5 mm IZD).
Conclusion: The non- sensitivity of the plethora of the cattle rumen waste bacteria to aqueous leaf extracts of\&nbsp;B.buonopozense\&nbsp;and\&nbsp;N. latifolia\&nbsp;may be indicative of their possible usefulness in bioconservation and environmental protection. This means that the use of cattle rumen waste as manure for economic vegetables may not pose additional threat to the ecosystem as the vegetable farm irrigation water bacterial flora were not sensitive to\&nbsp;B. buonopozense\&nbsp;and\&nbsp;N. latifolia.



Keywords: Bombacopsis buonopozense; Nauclea latifolia; FTIR spectroscopy; bioactive compounds; bacteria, antibacterial activity
},
	url = {http://journals.uniosun.edu.ng/index.php/ujs/article/view/13},
	author = {S. B. Akinde and E. I. O. Ajayi and O. I. Awojide and O. K. Azeez and K. O. Osunsanmi and O. A. Olabiran and A. A. Fowosire}
}
